An unique solvent assisted ‘green’ hydrotropic precipitation and response surface optimization for isolation of the dietary micronutrient β-sitosterol-d-glucopyranoside from Desmostachya bipinnata

Abstract

β-sitosterol-D-glucopyranoside, a phytosterol glycoside is well known as dietary micronutrient and food supplement. The present work focuses on the novel investigation of solvent assisted ‘green’ hydrotropic precipitation of this molecule from D. bipinnata, where the Box–Behnken optimized separation process improved the yield by 80.4% (purity: 99.6%) than the conventional extraction.