Macroporous adsorbent resin-based wheat bran polyphenol extracts inhibition effects on H2O2-induced oxidative damage in HEK293 cells

Abstract

In the present study, polyphenol-rich extracts of wheat bran (PEWB) were prepared via adsorption on macroporous resins and desorption with ethanol. Extraction was performed using aqueous ethanol and four different types of macroporous adsorbent resins for isolation. Specifically, the properties of the macroporous resins were investigated by adsorption and desorption tests. Total polyphenolic content of PEWB was determined using the Folin–Ciocalteu method, and its resistance effects against hydrogen peroxide (H₂O₂)-induced oxidation on HEK293 cells were assessed by cell viability and the reactive oxygen species (ROS) assay. The results indicate that resin NKA-9 displayed excellent adsorption and separation ability, as well as provided insight into the generation of PEWB from wheat bran extracts. In addition, these results suggest that pretreating HEK293 cells with PEWB prior to H₂O₂ exposure exhibited a significantly increased survival ratio and reduced the ROS levels. Further investigation involving the phenolic content of PEWB identification and quantification demonstrated that ferulic acid was the most abundant phenolic compound in a number of extracts, which was also confirmed with MTT and ROS assays. Our study revealed that PEWB can prevent HEK293 cells from H₂O₂-induced oxidative damage.