Issue 53, 2015

Fabrication and in vivo chondrification of a poly(propylene carbonate)/l-lactide-grafted tetracalcium phosphate electrospun scaffold for cartilage tissue engineering

Abstract

Regenerative therapies that utilize stem cell differentiation in three-dimensional porous scaffolds have attracted significant interest in recent years. In this study, fibrous poly(propylene carbonate)/poly(L-lactic acid)-grafted tetracalcium phosphate (PPC/g-TTCP) scaffolds were prepared using an electrospinning method. The characteristics of the fabricated scaffolds were investigated using scanning electron microscopy, differential scanning calorimetry, thermogravimetric analyses, Fourier transform infrared spectroscopy, X-ray diffraction analyses, water contact angle measurements and tensile tests. Due to the importance of biocompatibility, rat bone marrow-derived stem cells were cultured on the scaffolds, and the cell proliferation was investigated using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assays. Subsequently, chondrogenic differentiation was induced in these cells in vitro and in vivo. Fourteen days later, chondrocyte-like cells had developed on the PPC/g-TTCP scaffolds, as evidenced by the accumulation of glycosaminoglycan and type II collagen. After subcutaneous transplantation into nude mice, a typical cartilage cell morphology was observed on the scaffolds. These findings suggest that PPC/g-TTCP scaffolds can support cartilage development and are excellent candidate scaffolds for cartilage defect repair.

Graphical abstract: Fabrication and in vivo chondrification of a poly(propylene carbonate)/l-lactide-grafted tetracalcium phosphate electrospun scaffold for cartilage tissue engineering

Supplementary files

Article information

Article type
Paper
Submitted
13 Mar 2015
Accepted
08 May 2015
First published
08 May 2015

RSC Adv., 2015,5, 42943-42954

Fabrication and in vivo chondrification of a poly(propylene carbonate)/L-lactide-grafted tetracalcium phosphate electrospun scaffold for cartilage tissue engineering

J. Deng, Y. Wang, L. Zhou, M. Gou, N. Luo, H. Chen, A. Tong, C. You and G. Guo, RSC Adv., 2015, 5, 42943 DOI: 10.1039/C5RA04442A

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