Issue 104, 2015

A microfluidic capacitive immunosensor system for human cartilage chitinase-3-like protein 2 (hYKL-39) quantification as an osteoarthritis marker in synovial joint fluid

Abstract

Worldwide, many people suffer from osteoarthritis, a painful condition that interferes with normal work and the enjoyment of life and is thus a serious personal and public problem. Good management of osteoarthritis requires methodologies for accurate and timely detection of disease onset, progression and response to treatment. Here, capacitive electrochemical immunosensors, using immobilized antibodies against the known osteoarthritis biomarker human cartilage chitinase-3-like protein 2, also known as hYKL-39, were used as novel tools for sensitive and selective osteoarthritis analysis. In the proposed procedure the current response of the sensor to a potential step application is measured; from the resultant traces, concentration-dependent capacitance changes, due to antigen binding, can be calculated. The linear range and detection limit of capacitive hYKL-39 immunosensing were respectively 0.1–1000 μg L−1 and 0.074 μg L−1. This was better than the characteristics of the enzyme-linked immunosorbent assay (ELISA) with an optical readout, which is presently the standard practice for laboratory hYKL-39 screening. In synovial fluid samples from osteoarthritis patients, capacitive hYKL-39 immunosensing reported the expected elevated hYKL-39 biomarker levels in quantitative agreement with ELISA analysis. The improved detection limit and linear range of the electrochemical hYKL-39 immunoassay combined with the potential of cheap, simple, miniaturizable, portable and automatable equipment recommend this novel approach as a promising complementary tool and ideal reference assay for osteoarthritis diagnosis and monitoring, in particular when the aim is early detection.

Graphical abstract: A microfluidic capacitive immunosensor system for human cartilage chitinase-3-like protein 2 (hYKL-39) quantification as an osteoarthritis marker in synovial joint fluid

Supplementary files

Article information

Article type
Paper
Submitted
15 Jun 2015
Accepted
28 Sep 2015
First published
29 Sep 2015

RSC Adv., 2015,5, 85410-85416

Author version available

A microfluidic capacitive immunosensor system for human cartilage chitinase-3-like protein 2 (hYKL-39) quantification as an osteoarthritis marker in synovial joint fluid

W. Chaocharoen, A. Ranok, W. Suginta and A. Schulte, RSC Adv., 2015, 5, 85410 DOI: 10.1039/C5RA11379B

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