A novel colorimetric and near-infrared fluorescent probe for hydrogen peroxide imaging in vitro and in vivo

Abstract

A novel NIR fluorescent probe (DCM-B2) based on dicyanomethylene-4H-pyran was synthesized for the detection of H₂O₂. This colorimetric fluorescent probe displays a fluorescence turn-on response following the conversion of the aryl boronate unit to phenol in the presence of H₂O₂. It could offer good performance in terms of sensitivity, selectivity, and low cytotoxicity. Furthermore, bioimaging investigations indicated that this probe was cell permeable and suitable for monitoring H₂O₂ in vitro and in vivo.