Issue 130, 2015

Colourimetric assay for β-estradiol based on the peroxidase-like activity of Fe3O4@mSiO2@HP-β-CD nanoparticles

Abstract

Magnetic mesoporous nanoparticles of Fe3O4@mSiO2 were prepared through co-precipitation and tetraethoxysilane hydrolysis at 60 °C and pH 10 with hexadecyl trimethyl ammonium bromide as the template. Hydroxypropyl β-cyclodextrin was modified onto Fe3O4@mSiO2 under sodium citrate and N2 protection via an ultrasound process to increase the catalytic efficiency. The prepared nanoparticles were characterized by Fourier transform infrared spectrometry, X-ray diffraction, scanning electron microscopy and a peroxidase-like activity assay. The results showed that the prepared nanoparticles have magnetic properties, peroxidase-like activity and a loose spherical cluster structure. Compared with Fe3O4@mSiO2 nanoparticles, Fe3O4@mSiO2@HP-β-CD nanoparticles exhibit higher catalytic ability toward both H2O2 and 3,3′,5,5′-tetramethylbenzidine. Fe3O4@mSiO2@HP-β-CD nanoparticles can catalyse β-estradiol (β-E2) oxidation in the presence of H2O2 and be used as a colourimetric sensor for indirect detection of β-E2. A good linear relationship was obtained from 0.8 μM to 16 μM. The limit of detection of the proposed method was 0.2 μM. The visual method was successfully used in the analysis of β-E2 in commercial tablets and animal feeds, with recovery ranging from 92.6% to 110%.

Graphical abstract: Colourimetric assay for β-estradiol based on the peroxidase-like activity of Fe3O4@mSiO2@HP-β-CD nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
06 Oct 2015
Accepted
10 Dec 2015
First published
14 Dec 2015

RSC Adv., 2015,5, 107670-107679

Author version available

Colourimetric assay for β-estradiol based on the peroxidase-like activity of Fe3O4@mSiO2@HP-β-CD nanoparticles

S. Wei, J. Li and Y. Liu, RSC Adv., 2015, 5, 107670 DOI: 10.1039/C5RA20695B

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