Issue 1, 2015

Core–shell structured phosphorescent nanoparticles for detection of exogenous and endogenous hypochlorite in live cells via ratiometric imaging and photoluminescence lifetime imaging microscopy

Abstract

We report a ratiometric phosphorescence sensory system for hypochlorite (ClO) based on core–shell structured silica nanoparticles. Two phosphorescent iridium(III) complexes were immobilised in the inner solid core and outer mesoporous layer of the nanoparticles, respectively. The former is insensitive to ClO and thus serves as an internal standard to increase the accuracy and precision, while the latter exhibits a specific and significant luminogenic response to ClO, providing high selectivity and sensitivity. Upon exposure to ClO, the nanoparticles display a sharp luminescence colour change from blue to red. Additionally, intracellular detection of exogenous and endogenous ClO has been demonstrated via ratiometric imaging and photoluminescence lifetime imaging microscopy. Compared to intensity-based sensing, ratiometric and lifetime-based measurements are independent of the probe concentration and are thus less affected by external influences, especially in intracellular applications.

Graphical abstract: Core–shell structured phosphorescent nanoparticles for detection of exogenous and endogenous hypochlorite in live cells via ratiometric imaging and photoluminescence lifetime imaging microscopy

Supplementary files

Article information

Article type
Edge Article
Submitted
26 Aug 2014
Accepted
22 Sep 2014
First published
23 Sep 2014
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2015,6, 301-307

Core–shell structured phosphorescent nanoparticles for detection of exogenous and endogenous hypochlorite in live cells via ratiometric imaging and photoluminescence lifetime imaging microscopy

K. Y. Zhang, J. Zhang, Y. Liu, S. Liu, P. Zhang, Q. Zhao, Y. Tang and W. Huang, Chem. Sci., 2015, 6, 301 DOI: 10.1039/C4SC02600D

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