Issue 2, 2015

DNA cross-triggered cascading self-amplification artificial biochemical circuit

Abstract

The construction of compact and robust artificial biochemical circuits based on nucleic acids can help researchers to understand the essential mechanisms of complex biological systems, and design sophisticated strategies for various requirements. In this study, a novel DNA cross-triggered cascading self-amplification artificial biochemical circuit was developed. Once triggered by trace amounts (as low as 2 amol) of either of two fully independent oligonucleotide factors under homogeneous isothermal conditions, the circuit simultaneously amplified both factors by 105–107 fold, which was proved using mass spectrometry. The compact and robust circuit was successfully used to construct a multi-input Boolean logic operation and a sensitive DNA biosensor based on the dual-amplification of both the target and reporter. The circuit showed great potential for signal gain in complicated molecular programming, and flexible control of nucleic acid nanomachines in biochemical network systems and nanotechnology.

Graphical abstract: DNA cross-triggered cascading self-amplification artificial biochemical circuit

Supplementary files

Article information

Article type
Edge Article
Submitted
21 Oct 2014
Accepted
05 Nov 2014
First published
07 Nov 2014
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2015,6, 1225-1229

Author version available

DNA cross-triggered cascading self-amplification artificial biochemical circuit

J. Nie, M. Zhao, W. J. Xie, L. Cai, Y. Zhou and X. Zhang, Chem. Sci., 2015, 6, 1225 DOI: 10.1039/C4SC03225J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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