Biocompatible and antifouling coating of cell membrane phosphorylcholine and mussel catechol modified multi-arm PEGs

Abstract

The design and easy fabrication of biocompatible and antifouling coatings on different materials are extremely important for biotechnological and biomedical devices. Here we report a substrate-independent biomimetic modification strategy for fabricating a biocompatible and antifouling ultra-thin coating. Cell membrane antifouling phosphorylcholine (PC) and/or mussel adhesive catechol (c) groups are grafted at the amino-ends of an 8-armed poly(ethylene glycol). The PC groups are introduced by grafting a random copolymer bearing both PC and active ester groups. The modified 8-arm PEGs (PEG-2c-23PC, PEG-6c-23PC and PEG-8c) anchor themselves onto various substrates from aqueous solution and form cell outer membrane mimetic surfaces. Static contact angle, atomic force microscope (AFM) and X-ray photoelectron spectra (XPS) measurements confirm the successful fabrication of coatings on polydopamine (PDA) precoated surfaces. Real-time interaction results between proteins/bacteria and the coatings measured by surface plasmon resonance (SPR) technique suggest excellent anti-protein adsorption and short-term anti-bacteria adhesion performance. The long-term bacteria adhesion, platelet and L929 cell attachment results strongly support the SPR conclusions. Furthermore, the cell membrane mimetic and mussel adhesive protein mimetic PEG-2c-23PC shows hardly any toxicity to L929 fibroblasts, and the coating surface demonstrates the best anti-biofouling performance. This PDA-assisted immobilization of PC and/or catechol modified multi-arm PEGs provides a convenient and universal way to produce a biocompatible and fouling-resistant surface with tailor-made functions, which hopefully can be expanded to a wider range of applications based on both structure and surface superiorities.