Issue 8, 2016
From the journal:

Analyst

Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions†

Taeseok Oh,a   Jae-Young Choib  and  Michael J. Heller*bc  

* Corresponding authors

a Materials Science and Engineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA

b Department of NanoEngineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
E-mail: mheller@ucsd.edu

c Department of BioEngineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA

Abstract

Dimerization and resultant quenching of donor and acceptor dyes conjugated on DNA causes loss of fluorescent resonant energy transfer (FRET) efficiency. However, when complexed with surfactants and divalent metal ions, sheathing effects insulate and shield the DNA structures, reducing dimerization and quenching which leads to significant enhancement of FRET efficiency.

Graphical abstract: Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions

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Article information

DOI
https://doi.org/10.1039/C5AN02516H
Article type
Communication
Submitted
07 Dec 2015
Accepted
08 Mar 2016
First published
08 Mar 2016

Analyst, 2016,141, 2371-2375

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Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions

T. Oh, J. Choi and M. J. Heller, Analyst, 2016, 141, 2371 DOI: 10.1039/C5AN02516H

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