Issue 13, 2016

Integration of a guided-mode resonance filter with microposts for in-cell protein detection

Abstract

We present an integrated microfluidic system consisting of a label-free biosensor of a guided-mode resonance filter (GMRF) and a microfluidic channel with a micropost filter. The GMRF was fabricated through replica molding using an ultraviolet-curable polymer and a plastic substrate. An array of microposts (a diameter and height of 26.5 and 56 μm, respectively, and a spacing between 7.5 and 9.5 μm), fabricated on a silicon substrate through photolithography, was used as the filter. A double-sided tape was used to laminate the GMRF and a microfluidic chip such that the integrated device provides two functions: filtration of the cell debris and quantification of the in-cell protein concentration. By measuring the changes in the resonant wavelength from the GMRF, the detection of β-actin in an unprocessed lysed cell sample was demonstrated; the cell debris was separated using the micropost filter to prevent false measurement.

Graphical abstract: Integration of a guided-mode resonance filter with microposts for in-cell protein detection

Supplementary files

Article information

Article type
Paper
Submitted
05 Jan 2016
Accepted
29 Apr 2016
First published
29 Apr 2016

Analyst, 2016,141, 4189-4195

Integration of a guided-mode resonance filter with microposts for in-cell protein detection

Y. Tu, M. Tsai, I. Lee, H. Hsu and C. Huang, Analyst, 2016, 141, 4189 DOI: 10.1039/C6AN00023A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements