Issue 16, 2016

High-throughput metabolomics to identify metabolites to serve as diagnostic biomarkers of prostate cancer

Abstract

Prostate cancer (PCa) has long been known to exhibit unique metabolite profiles. Advances in liquid chromatography mass spectrometry technology have led to the application of metabolomic profiling to PCa toward identifying metabolic alterations in PCa that may provide clinically useful biomarkers. In this work, a metabolomics platform comprised of fast ultrahigh performance liquid chromatography-tandem mass spectrometry (FPLC/MS) coupled with multivariate statistical analyses was employed to identify the serum biomarker(s) associated with the entire measurable metabolome from PCa patients and age-matched healthy controls. Metabolic differences among PCa and control subjects were identified based on orthogonal signal correction-partial least squares discriminant analysis. 2-Isopropyl citrate, cytidine, D-asparagine, N-acetylgalactosamine-4-sulphate, 5-hydroxy-N-formylkynurenine and D-4-O-methyl-myo-inositol in the PCa subjects were significantly different from the control cases. To demonstrate the utility of serum biomarkers for the early diagnosis of PCa, three metabolites comprising 2-isopropyl citrate, cytidine and D-asparagine were selected as candidate biomarkers (AUC > 0.95) and validation in independent patient cohorts yielded satisfactory sensitivity. Furthermore, these data suggest that panels of analytes may be valuable to translate metabolomic findings to clinically useful diagnostic tests. Potentially, the present study provides the diagnosis tool for PCa in its early stage.

Graphical abstract: High-throughput metabolomics to identify metabolites to serve as diagnostic biomarkers of prostate cancer

Supplementary files

Article information

Article type
Communication
Submitted
14 Jan 2016
Accepted
19 Mar 2016
First published
22 Mar 2016

Anal. Methods, 2016,8, 3284-3290

High-throughput metabolomics to identify metabolites to serve as diagnostic biomarkers of prostate cancer

Y. Li, S. Qiu and A. H. Zhang, Anal. Methods, 2016, 8, 3284 DOI: 10.1039/C6AY00127K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements