Issue 28, 2016

Evaluation of procedures for assessing anti- and pro-oxidants in plant samples

Abstract

Plants as well as other aerobic organisms constantly produce reactive oxygen species (ROS). At regulated low concentrations ROS may serve as signal molecules, while in excessive amounts these may cause oxidative damage to biomolecules. Actual cellular concentrations are controlled by a network of various antioxidants, and acclimation to stress conditions is achieved by a dynamic balance of ROS production and neutralization. Accordingly, plant stress physiology studies generally include an array of methods testing the occurrence of ROS as well as evaluating antioxidant capacities. The aim of the present work is to provide an overview of these methods, with special emphasis on avoiding errors that can possibly lead to either inaccurate data or misinterpretations of otherwise correct measurements.

Graphical abstract: Evaluation of procedures for assessing anti- and pro-oxidants in plant samples

Article information

Article type
Critical Review
Submitted
09 May 2016
Accepted
13 Jun 2016
First published
15 Jun 2016

Anal. Methods, 2016,8, 5569-5580

Evaluation of procedures for assessing anti- and pro-oxidants in plant samples

P. Majer, M. Vidović, Gy. Czégény, S. Veljović Jovanović, Å. Strid and É. Hideg, Anal. Methods, 2016, 8, 5569 DOI: 10.1039/C6AY01342B

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