Engineered variants of the capsid-forming enzyme lumazine synthase, AaLS, were used as nanoreactors for an enzyme-mediated polymerization. Oxidation of 3,3-diaminobenzidine (DAB) by the engineered ascorbate peroxidase APEX2 encapsulated in AaLS capsids resulted in templated formation of polyDAB–capsid nanoparticles of homogeneous size and shape.
R. Frey, T. Hayashi and D. Hilvert, Chem. Commun., 2016, 52, 10423 DOI: 10.1039/C6CC05301G
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