A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors

Abstract

We present a new platform for multiplexed protein kinase activity assay using TiO₂ decorated graphene oxide (GO), which is applicable to high throughput inhibitor screening. On the basis of the strong affinity of TiO₂ for the phosphate group and the fluorescence quenching capability of GO, phosphorylation of substrates by protein kinases was quantitatively measured in a short time.