Issue 98, 2016

A chemical proteomic probe for detecting native carrier protein motifs in nonribosomal peptide synthetases

Abstract

Derivatization of a 5′-(vinylsulfonylaminodeoxy)adenosine scaffold with a clickable functionality provided an activity-based probe that was used to label native carrier protein (CP) motifs in nonribosomal peptide synthetases (NRPSs). When coupled with a fluorescent tag, this probe selectively targeted phosphopantetheinylated CPs (holo-form) from recombinant NRPS enzyme systems and in whole proteomes.

Graphical abstract: A chemical proteomic probe for detecting native carrier protein motifs in nonribosomal peptide synthetases

Supplementary files

Article information

Article type
Communication
Submitted
26 Sep 2016
Accepted
10 Nov 2016
First published
11 Nov 2016

Chem. Commun., 2016,52, 14129-14132

A chemical proteomic probe for detecting native carrier protein motifs in nonribosomal peptide synthetases

S. Kasai, F. Ishikawa, T. Suzuki, N. Dohmae and H. Kakeya, Chem. Commun., 2016, 52, 14129 DOI: 10.1039/C6CC07793E

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