Issue 6, 2016

Intestinal immunomodulating activity and structural characterization of a new polysaccharide from stems of Dendrobium officinale

Abstract

A homogeneous polysaccharide fraction (DOP-W3-b) with a high intestinal immunomodulating activity was obtained from the stems of Dendrobium officinale through a bioactivity-guided sequential isolation procedure based on the screening of Peyer's patch-mediated immunomodulating activity. Oral administration experiments of mice showed that DOP-W3-b could effectively regulate intestinal mucosal immune activity by changing intestinal mucosal structures, promoting the secretions of cytokines from Peyer's patches (PPs) and mesenteric lymph nodes (MLNs), and increasing the production of secretory immunoglobulin A (sIgA) in the lamina propria. Structure analysis indicated that DOP-W3-b was composed of mannose and glucose in a molar ratio of 4.5 with a relatively low molecular weight of 1.543 × 104 Da, and its repeat unit contained a backbone consisting of β-(1→4)-D-Manp, β-(1→4)-D-Glcp and β-(1→3,6)-D-Manp residues, a branch consisting of β-(1→4)-D-Manp, β-(1→4)-D-Glcp and terminal β-D-Glcp, and O-acetyl groups attached to O-2 of β-(1→4)-D-Manp. These results suggested that DOP-W3-b was a new polysaccharide with an essential potential for modulating body's immune functions.

Graphical abstract: Intestinal immunomodulating activity and structural characterization of a new polysaccharide from stems of Dendrobium officinale

Supplementary files

Article information

Article type
Paper
Submitted
08 Feb 2016
Accepted
09 May 2016
First published
12 May 2016

Food Funct., 2016,7, 2789-2799

Author version available

Intestinal immunomodulating activity and structural characterization of a new polysaccharide from stems of Dendrobium officinale

S. Xie, B. Liu, D. Zhang, X. Zha, L. Pan and J. Luo, Food Funct., 2016, 7, 2789 DOI: 10.1039/C6FO00172F

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