Extruded whole grain diets based on brown, soaked and germinated rice. Effects on cecum health, calcium absorption and bone parameters of growing Wistar rats. Part I

Abstract

The influence of diets with whole rice processed ingredients on cecum health, calcium absorption and bone parameters was studied using an animal model. Thirty-two male Wistar rats were fed with Control (C), extruded Brown rice (B), extruded Soaked whole rice (S) and extruded Germinated whole rice (G) diets for 60 days. The cecum weight, cecal content pH, cecal sIgA content, and β-glucosidase and β-glucuronidase activities were determined. Calcium apparent absorption, total bone mineral content and density and right femur parameters (ashes, organic content, calcium and P) were evaluated. The results showed that animals fed with whole grain diets have lower food intake in comparison with the C diet, and decreased cecal content pH (7.06 vs. 6.33) and β-glucosidase activity (1.66 vs. 0.21 μmol p-nitrophenol g⁻¹ cc h⁻¹). Even though calcium apparent absorption was not different among treatments (∼70%), none of the whole grain diets improved calcium related bone parameters over the control fed rats (cellulose as dietary fibre).