Genetic incorporation of 1,2-aminothiol functionality for site-specific protein modification via thiazolidine formation†
Abstract
Here we report a new site-specific conjugation strategy to modify proteins via thiazolidine ligation. Proteins harbouring a 1,2-aminothiol moiety introduced by amber codon suppression technology could be modified chemoselectively with aldehyde-functionalized reagents, such as a biotin-labeled peptide or ubiquitin, under mild conditions to yield homogeneous biotinylated or ubiquitinated products.
- This article is part of the themed collection: Selective Chemistry with Peptides and Proteins