An improved miniprotein host capable of fluorogenic supramolecular assembly with a sI-Pht guest is reported. This improved host, termed 6.2.22, displays significant enhancements in both the EC50 for complexation and fluorescence activation of the sI-Pht guest, allowing for improved resolution of supramolecular complexation on the surface of living yeast.
B. Xu, X. Zhou and C. I. Stains, RSC Adv., 2016, 6, 20381 DOI: 10.1039/C6RA01215A
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