Optimization of magnetic hyperthermia effect for breast cancer stem cell therapy

Abstract

We successfully prepared magnetic nanoclusters (MNCs) with regular size distribution and high solution stability. To increase the therapeutic effectiveness of MNCs, various factors such as the size distribution of particles, external field frequency and strength should be optimized. The correlation between heat generation ability and the various factors was evaluated by measuring the specific absorption rate. The 60 nm MNC showed the highest heat capacity ability in the various field conditions. Breast cancer stem cells (bCSCs) with chemo/radio-resistance were exposed to the optimized alternating magnetic field after treating with the MNC. The MNC promoted an appropriate temperature increase within 10 min and the particles influenced directly heat shock protein 70 and 90 damage during the magnetic hyperthermia. Thus, the treated CSCs were progressed to the thermal-mediated apoptotic cell death. Our optimization for magnetic hyperthermia will be useful for a variety of research on cancer cell therapy.