Issue 16, 2016

Modulating in vivo degradation rate of injectable extracellular matrix hydrogels

Abstract

Extracellular matrix (ECM) derived hydrogels are increasingly used as scaffolds to stimulate endogenous repair. However, few studies have examined how altering the degradation rates of these materials affect cellular interaction in vivo. This study sought to examine how crosslinking or matrix metalloproteinase (MMP) inhibition by doxycycline could be employed to modulate the degradation rate of an injectable hydrogel derived from decellularized porcine ventricular myocardium. While both approaches were effective in reducing degradation in vitro, only doxycycline significantly prolonged hydrogel degradation in vivo without affecting material biocompatibility. In addition, unlike crosslinking, incorporation of doxycycline into the hydrogel did not affect mechanical properties. Lastly, the results of this study highlighted the need for development of novel crosslinkers for in situ modification of injectable ECM-derived hydrogels, as none of the crosslinking agents investigated in this study were both biocompatible and effective.

Graphical abstract: Modulating in vivo degradation rate of injectable extracellular matrix hydrogels

Article information

Article type
Paper
Submitted
07 Dec 2015
Accepted
24 Mar 2016
First published
28 Mar 2016

J. Mater. Chem. B, 2016,4, 2794-2802

Author version available

Modulating in vivo degradation rate of injectable extracellular matrix hydrogels

J. W. Wassenaar, R. L. Braden, K. G. Osborn and K. L. Christman, J. Mater. Chem. B, 2016, 4, 2794 DOI: 10.1039/C5TB02564H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements