Issue 7, 2016

Fluorinated dendrimer for TRAIL gene therapy in cancer treatment

Abstract

The delivery of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) gene into cancer cells is a promising strategy for cancer treatment. However, the low transfection efficacy and/or the toxicity of vectors severely hamper the translation of TRAIL gene therapy into the clinics. In this article, we employed our recently developed fluorinated dendrimer as a vector to deliver plasmid encoding TRAIL (pTRAIL) into cancer cells for cancer treatment, which holds the advantages of both excellent transfection efficacy and low toxicity. Fluorinated poly(amidoamine) dendrimer (G4-F735) represented much higher TRAIL gene transfection efficacy than a series of transfection reagents including poly(ethylene imine) (PEI), SuperFect and Lipofectamine 2000, leading to a much higher cell apoptosis efficacy. The G4-F735/pTRAIL complex, compared with PEI/pTRAIL, could more efficiently destroy three-dimensional multicellular spheroids consisting of MDA-MB-231 cells, and suppress the tumor growth in vivo. Furthermore, G4-F735 showed minimal toxicity in vitro and undetectable systemic toxicity in vivo. From this study, the fluorinated dendrimer offers a promising prospect for TRAIL gene therapy.

Graphical abstract: Fluorinated dendrimer for TRAIL gene therapy in cancer treatment

Supplementary files

Article information

Article type
Paper
Submitted
22 Dec 2015
Accepted
17 Jan 2016
First published
18 Jan 2016

J. Mater. Chem. B, 2016,4, 1354-1360

Author version available

Fluorinated dendrimer for TRAIL gene therapy in cancer treatment

Y. Wang, M. Wang, H. Chen, H. Liu, Q. Zhang and Y. Cheng, J. Mater. Chem. B, 2016, 4, 1354 DOI: 10.1039/C5TB02712H

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