Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity

Suvendu Biswas; Brandon S. McCullough; Elena S. Ma; Dollie LaJoie; Colin W. Russell; D. Garrett Brown; June L. Round; Katharine S. Ullman; Matthew A. Mulvey; Amy M. Barrios

doi:10.1039/C6CC09204G

Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity†

Suvendu Biswas,^a Brandon S. McCullough,

^a Elena S. Ma,^a Dollie LaJoie,^b Colin W. Russell,

^c D. Garrett Brown,^c June L. Round,^c Katharine S. Ullman,^b Matthew A. Mulvey^c and Amy M. Barrios

*^a

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* Corresponding authors

^a Department of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, Utah 84112, USA
E-mail: amy.barrios@utah.edu

^b Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA

^c Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA

Abstract

Two resorufin-based substrates for protein tyrosine phosphatase (PTP) activity have been synthesized. These substrates provide sensitive fluorogenic readouts of PTP activity in vitro and in living cells at both acidic and neutral pH. In addition, the presence of the pathogenic bacteria Staphylococcus aureus was detected visually using a colorimetric readout.

Chemical Communications

Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity†

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Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity

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