Issue 23, 2017

Mass spectrometry captures structural intermediates in protein fiber self-assembly

Abstract

Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process.

Graphical abstract: Mass spectrometry captures structural intermediates in protein fiber self-assembly

Supplementary files

Article information

Article type
Communication
Submitted
13 Jan 2017
Accepted
01 Feb 2017
First published
01 Feb 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 3319-3322

Mass spectrometry captures structural intermediates in protein fiber self-assembly

M. Landreh, M. Andersson, E. G. Marklund, Q. Jia, Q. Meng, J. Johansson, C. V. Robinson and A. Rising, Chem. Commun., 2017, 53, 3319 DOI: 10.1039/C7CC00307B

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