Issue 48, 2017
From the journal:

Chemical Communications

A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity†

Shuang Leng,‡ab   Qinglong Qiao,‡ac   Lu Miao,*a   Wuguo Deng,*bd   Jingnan Cui*c  and  Zhaochao Xu ORCID logo *a  

* Corresponding authors

a Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
E-mail: zcxu@dicp.ac.cn

b Institute of Cancer Stem Cell, Cancer Center, Dalian Medical University, Dalian, China

c State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China
E-mail: jncui@dlut.edu.cn

d Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, China
E-mail: dengwg@sysucc.org.cn

Abstract

A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O6-benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.

Graphical abstract: A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity

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Article information

DOI
https://doi.org/10.1039/C7CC01483J
Article type
Communication
Submitted
02 Mar 2017
Accepted
23 May 2017
First published
23 May 2017

Chem. Commun., 2017,53, 6448-6451

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A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity

S. Leng, Q. Qiao, L. Miao, W. Deng, J. Cui and Z. Xu, Chem. Commun., 2017, 53, 6448 DOI: 10.1039/C7CC01483J

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