A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

Kondapa Naidu Bobba; Miae Won; Inseob Shim; Nithya Velusamy; Zhigang Yang; Junle Qu; Jong Seung Kim; Sankarprasad Bhuniya

doi:10.1039/C7CC05043G

A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells†

Kondapa Naidu Bobba,

‡^a Miae Won,

‡^b Inseob Shim,

‡^b Nithya Velusamy,

^a Zhigang Yang,

^c Junle Qu,

*^c Jong Seung Kim

*^bc and Sankarprasad Bhuniya

*^ad

Author affiliations

* Corresponding authors

^a Amrita Centre for Industrial Research & Innovation, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Ettimadai, Coimbatore 641112, India
E-mail: b_sankarprasad@cb.amrita.edu

^b Department of Chemistry, Korea University, Seoul 02841, Korea
E-mail: jongskim@korea.ac.kr

^c Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
E-mail: jlqu@szu.edu.cn

^d Department of Chemical Engineering and Materials Science, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Ettimadai, Coimbatore 641112, India

Abstract

Herein, we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference of other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λ_em = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 provides information about H₂O₂-mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells.

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Article information

DOI: https://doi.org/10.1039/C7CC05043G
Article type: Communication
Submitted: 29 Jun 2017
Accepted: 10 Sep 2017
First published: 11 Sep 2017

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Chem. Commun., 2017,53, 11213-11216

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A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

K. Naidu Bobba, M. Won, I. Shim, N. Velusamy, Z. Yang, J. Qu, J. S. Kim and S. Bhuniya, Chem. Commun., 2017, 53, 11213 DOI: 10.1039/C7CC05043G

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Chemical Communications

A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells†

Abstract

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A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

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