Triazolium cyclodextrin click cluster–resin conjugate: an enrichment material for phosphatidylinositol (3,4,5)-triphosphate†
Abstract
UltraLink was functionalized with a triazolium cyclodextrin click cluster (CCC) which provides a well-oriented, multivalent, positively charged binding site for PtdIns(3,4,5)P3. MALDI TOF MS and LC ESI MS/MS MRM analysis of spiked PtdIns(3,4,5)P3 in lipid extract suggest that triazolium CCC–UltraLink conjugate can be used as an enrichment material for PtdIns(3,4,5)P3.