Issue 87, 2017

Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

Abstract

A label-free and general thermo-controlled split apta-PCR strategy was first developed for the sensitive and specific detection of cancer cells. By integrating the temperature-responsive function of split aptamers with PCR amplification, a facile fluorescence assay of liver cancer SMMC-7721 cells was successfully realized with the detection of as low as 100 cells.

Graphical abstract: Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

Supplementary files

Article information

Article type
Communication
Submitted
09 Aug 2017
Accepted
10 Oct 2017
First published
10 Oct 2017

Chem. Commun., 2017,53, 11889-11892

Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

J. Tang, X. He, Y. Lei, H. Shi, Q. Guo, J. Liu, D. He, L. Yan and K. Wang, Chem. Commun., 2017, 53, 11889 DOI: 10.1039/C7CC06218D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements