Surface charge tuneable fluorescent protein-based logic gates for smart delivery of nucleic acids

Abstract

A versatile bio-logic system is proposed using a surface charge tunable fluorescent protein (H₃₉GFP) as a generic processor. On the basis of the cell penetration of H₃₉GFP triggered by H⁺ and metal ions, OR logic gates are successfully operated in living cells with transfection of functional nucleic acids (Cy5 labelled DNA and siRNA) as the readout, as well as RNA interference.