Issue 26, 2017

An efficient enzyme-powered micromotor device fabricated by cyclic alternate hybridization assembly for DNA detection

Abstract

An efficient enzyme-powered micromotor device was fabricated by assembling multiple layers of catalase on the inner surface of a poly(3,4-ethylenedioxythiophene and sodium 4-styrenesulfonate)/Au microtube (PEDOT-PSS/Au). The catalase assembly was achieved by programmed DNA hybridization, which was performed by immobilizing a designed sandwich DNA structure as the sensing unit on the PEDOT-PSS/Au, and then alternately hybridizing with two assisting DNA to bind the enzyme for efficient motor motion. The micromotor device showed unique features of good reproducibility, stability and motion performance. Under optimal conditions, it showed a speed of 420 μm s−1 in 2% H2O2 and even 51 μm s−1 in 0.25% H2O2. In the presence of target DNA, the sensing unit hybridized with target DNA to release the multi-layer DNA as well as the multi-catalase, resulting in a decrease of the motion speed. By using the speed as a signal, the micromotor device could detect DNA from 10 nM to 1 μM. The proposed micromotor device along with the cyclic alternate DNA hybridization assembly technique provided a new path to fabricate efficient and versatile micromotors, which would be an exceptional tool for rapid and simple detection of biomolecules.

Graphical abstract: An efficient enzyme-powered micromotor device fabricated by cyclic alternate hybridization assembly for DNA detection

Supplementary files

Article information

Article type
Paper
Submitted
16 Feb 2017
Accepted
28 May 2017
First published
02 Jun 2017

Nanoscale, 2017,9, 9026-9033

An efficient enzyme-powered micromotor device fabricated by cyclic alternate hybridization assembly for DNA detection

S. Fu, X. Zhang, Y. Xie, J. Wu and H. Ju, Nanoscale, 2017, 9, 9026 DOI: 10.1039/C7NR01168G

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