Issue 1, 2017

Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2–8-sialosides and high-throughput substrate specificity studies of α2–8-sialidases

Abstract

para-Nitrophenol (pNP)-tagged α2–8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme α2–8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the α2–8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglycan analysis and for cell and tissue surface glycan modification. They can also be used to guide sialidase inhibitor design.

Graphical abstract: Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2–8-sialosides and high-throughput substrate specificity studies of α2–8-sialidases

Supplementary files

Article information

Article type
Paper
Submitted
14 Oct 2016
Accepted
24 Nov 2016
First published
24 Nov 2016

Org. Biomol. Chem., 2017,15, 160-167

Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2–8-sialosides and high-throughput substrate specificity studies of α2–8-sialidases

N. Tasnima, H. Yu, Y. Li, A. Santra and X. Chen, Org. Biomol. Chem., 2017, 15, 160 DOI: 10.1039/C6OB02240E

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