Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage

Getong Liu; Xin Huang; Qinlin Pu; Yongyun Zhao; Feng Du; Juan Dong; Xin Cui; Zhuo Tang

doi:10.1039/C7OB00995J

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Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage†

Getong Liu,^a Xin Huang,^a Qinlin Pu,^a Yongyun Zhao,^a Feng Du,^a Juan Dong,^a Xin Cui^a and Zhuo Tang

*^a

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* Corresponding authors

^a Natural Products Research Center Chengdu Institution of Biology, University of Chinese Academy of Science, Chengdu, Sichuan, P.R. China
E-mail: tangzhuo@cib.ac.cn

Abstract

A new guideline for the construction of hammerhead ribozymes to achieve trans-cleavage of a single-stranded RNA molecule was developed. The sequence rule of the HHRz cleavage site was highly recommended to be “DWH” with an optimal binding arm length of 8–9nt, which diverged from the former rule of “NUX”.

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DOI: https://doi.org/10.1039/C7OB00995J
Article type: Paper
Submitted: 24 Apr 2017
Accepted: 09 May 2017
First published: 09 May 2017

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Org. Biomol. Chem., 2017,15, 4681-4685

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Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage

G. Liu, X. Huang, Q. Pu, Y. Zhao, F. Du, J. Dong, X. Cui and Z. Tang, Org. Biomol. Chem., 2017, 15, 4681 DOI: 10.1039/C7OB00995J

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Organic & Biomolecular Chemistry

Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage†

Abstract

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Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage

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