Photo-induced DNA cleavage by zinc-substituted myoglobin with a redesigned active center

Abstract

Myoglobin with a redesigned active center was constructed and reconstituted with zinc protoporphyrin (ZnPP), which was shown to exhibit novel photo-induced DNA cleavage activities, involving a hydroxyl radical (˙OH) with a minor contribution from singlet oxygen (¹O₂), as confirmed by spin-trapping studies for the first time.