Issue 28, 2017, Issue in Progress

Protective effects of Shanxi aged vinegar against hydrogen peroxide-induced oxidative damage in LO2 cells through Nrf2-mediated antioxidant responses

Abstract

Shanxi aged vinegar (SAV), a kind of typical fermented food, is one of the famous traditional vinegars in China. In this study, the antioxidant properties and hepatoprotective mechanisms of SAV on hydrogen peroxide (H2O2)-induced oxidative stress were investigated. Total phenolic, flavonoid contents and browning index were increased during the aging process. Gallic acid was the main component of the phenolic compounds in SAV-5. SAV-5 showed the highest antioxidant activities among all the samples. In addition, H2O2 was used to treat human normal hepatocyte LO2 as an inducer of oxidative stress models. Pretreatment with SAV-5 inhibited H2O2-induced cytotoxicity and reactive oxygen species (ROS) generation, and alleviated loss of mitochondrial membrane potential in LO2 cells. These pretreatments also significantly increased superoxide dismutase (SOD), catalase (CAT) activities and glutathione (GSH) levels. The results demonstrated that SAV-5 had a significant antioxidant effect, which could prevent oxidative damage in LO2 cells effectively. Finally, SAV-5 activated nuclear factor erythroid-2-related factor 2 (Nrf2) and up-regulated NAD(P)H quinone oxidoreductase 1 (NQO1) and heme oxygenase (HO-1) protein expression in H2O2-treated LO2 cells. The protective effects of SAV against H2O2-induced oxidative damage in LO2 cells are mainly mediated by the activation of the Nrf2 antioxidant pathway. This study explored the fermented food products with antioxidant activities and the possible hepatoprotective mechanism of traditional Chinese vinegar against oxidative damage in liver cells.

Graphical abstract: Protective effects of Shanxi aged vinegar against hydrogen peroxide-induced oxidative damage in LO2 cells through Nrf2-mediated antioxidant responses

Article information

Article type
Paper
Submitted
05 Dec 2016
Accepted
01 Mar 2017
First published
20 Mar 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 17377-17386

Protective effects of Shanxi aged vinegar against hydrogen peroxide-induced oxidative damage in LO2 cells through Nrf2-mediated antioxidant responses

T. Xia, J. Yao, J. Zhang, Y. Zheng, J. Song and M. Wang, RSC Adv., 2017, 7, 17377 DOI: 10.1039/C6RA27789F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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