Issue 26, 2017, Issue in Progress

An antimicrobial peptide-based colorimetric bioassay for rapid and sensitive detection of E. coli O157:H7

Abstract

Rapid and sensitive detection of foodborne pathogens can effectively prevent or reduce the outbreaks of foodborne diseases. In this study, an antimicrobial peptide (AMP)-based colorimetric bioassay was developed for rapid and sensitive detection of Escherichia coli O157:H7. AMP was conjugated with horseradish peroxidase (AMP–HRP) to develop a signal reporter instead of antibody-HRP with a higher sensitivity. AMP–HRP could anchor on the surface of target bacteria rapidly through electrostatic and hydrophobic interactions. After unbound probes were removed by filtration, HRP catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB) to colored product, which was measured using UV-vis spectroscopy to quantify E. coli O157:H7. Due to the abundant AMP-binding sites on the surface of target bacteria, the proposed bioassay could detect E. coli O157:H7 as low as 13 cfu mL−1 in a pure culture with a linear range of 102–105 cfu mL−1 in 45 min without pre-enrichment. Combined with an immunomagnetic capture-release procedure, sensitive and selective detection of E. coli O157:H7 in food samples was achieved.

Graphical abstract: An antimicrobial peptide-based colorimetric bioassay for rapid and sensitive detection of E. coli O157:H7

Supplementary files

Article information

Article type
Paper
Submitted
18 Dec 2016
Accepted
04 Mar 2017
First published
10 Mar 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 15769-15775

An antimicrobial peptide-based colorimetric bioassay for rapid and sensitive detection of E. coli O157:H7

Z. Qiao, C. Lei, Y. Fu and Y. Li, RSC Adv., 2017, 7, 15769 DOI: 10.1039/C6RA28362D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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