Pharmacokinetics study of isorhamnetin in rat plasma by a sensitive electrochemical sensor based on reduced graphene oxide

Abstract

Isorhamnetin (ISO), a flavonol aglycone, plays an important role in human health, such as cardiovascular protection, anti-allergy activity, anti-inflammatory effects, and anticancer activity. Thus, the highly sensitive and rapid detection of ISO, and its pharmacokinetic study, has important clinical significance in the field of medicine. Herein, a simple, rapid, sensitive, and accurate method for the electrochemical determination of isorhamnetin (ISO) for pharmaceutical quality control and pharmacokinetic research was developed using an electrochemically reduced graphene oxide modified glassy carbon electrode (ERGO/GCE). The results indicated that the ERGO/GCE remarkably increased the peak currents of ISO oxidation. Under optimized conditions, using differential pulse voltammetry (DPV), the peak current had a linear dependence on the concentration of ISO in the range of 1.0 × 10⁻⁸ to 1.2 × 10⁻⁵ M with a low detection limit of 3.2 × 10⁻⁹ M. The present voltammetric method has been successfully applied to the detection of ISO in tablets and in plasma. Moreover, this assay has also been successfully applied in the pharmacokinetic study of ISO in vivo through intragastric administration of tablets to SD-rats. Therefore, this strategy opens up a new avenue for the application of an electrochemical method in the field of ISO pharmacokinetics.