Issue 63, 2017

Solid liquid liquid extraction of porcine gastric mucins from homogenized animal material

Abstract

Mucins purified from porcine stomachs have gained importance in biomedical applications as they exhibit unique features such as hydrogel formation, lubricity and antivirality. Because commercially available porcine gastric mucins (PGM) are neither gel forming, nor do they reduce friction, a robust purification process for functional mucins from porcine tissue (mainly Muc5AC) is necessary. Based on our former investigations (V. J. Schömig, B. T. Käsdorf, C. Scholz, K. Bidmon, O. Lieleg and S. Berensmeier, RSC Adv., 2016, 6, 44932–44943), we further optimized the established purification process in terms of productivity and overall yield as well as ease of scalability. We therefore introduced a novel extraction method – solid liquid liquid extraction (SLLE) – as an early capture step from homogenized porcine stomach, which combines conventional solid liquid extraction with a second, immiscible solvent, to simultaneously delipidate the tissue and extract hydrophilic proteins into the polar phase. Using Design of Experiments (DoE) the parameters incubation time and ratio of solvent phases (hexane/water) were identified to 3 h and 1/15 for SLLE, respectively. PGM was collected in the polar phase and further purified by size exclusion chromatography and diafiltration. With the homogenization of porcine stomach and the introduction of the SLLE, up to 3570 mg mucin per stomach can be purified, resulting in 55 times more PGM compared to the former published reference process. The productivity of the process was up to 25 mg mucin per stomach and per h, representing an improved process productivity by a factor of 4. Lubricity and gel formation of the purified mucin were retained with the optimized extraction protocol.

Graphical abstract: Solid liquid liquid extraction of porcine gastric mucins from homogenized animal material

Supplementary files

Article information

Article type
Paper
Submitted
13 Jun 2017
Accepted
07 Aug 2017
First published
14 Aug 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 39708-39717

Solid liquid liquid extraction of porcine gastric mucins from homogenized animal material

V. Schoemig, E. Isik, L. Martin and S. Berensmeier, RSC Adv., 2017, 7, 39708 DOI: 10.1039/C7RA06594A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements