Issue 61, 2017
From the journal:

RSC Advances

Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening†

Keitou Shu,ab   Taro Noguchi,a   Kaori Honda,c   Yasumitsu Kondoh,c   Hiroyuki Osada,c   Hiroaki Ohno,a   Nobutaka Fujiia  and  Shinya Oishi ORCID logo *a  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
E-mail: soishi@pharm.kyoto-u.ac.jp
Fax: +81-75-753-4570
Tel: +81-75-753-4561

b Graduate School of Advanced Integrated Studies in Human Survivability, Kyoto University, Sakyo-ku, Kyoto 606-8306, Japan

c Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan

Abstract

The Src SH2 domain was synthesized via native chemical ligation of two fragment peptides. The facile protocol was used to prepare the mirror-image SH2 domain (D-Src SH2 domain) and tetramethylrhodamine-labeled SH2 domains. The synthesized SH2 domains were correctly folded and showed activity, and using these proteins we established bioassays to identify novel Src SH2 domain inhibitors from an unexplored mirror-image library of natural products.

Graphical abstract: Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening

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Article information

DOI
https://doi.org/10.1039/C7RA07445J
Article type
Paper
Submitted
06 Jul 2017
Accepted
28 Jul 2017
First published
07 Aug 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 38725-38732

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Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening

K. Shu, T. Noguchi, K. Honda, Y. Kondoh, H. Osada, H. Ohno, N. Fujii and S. Oishi, RSC Adv., 2017, 7, 38725 DOI: 10.1039/C7RA07445J

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