Issue 81, 2017, Issue in Progress

Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles

Abstract

The styrene–maleic acid (SMA) copolymers enable a detergent-free extraction of membrane proteins from lipid bilayers yielding stable water-soluble nanocontainers with lipid-encased proteins. Here, we demonstrate the ability of the SMA copolymer to extract a complex of interacting membrane proteins from proteoliposomes, namely the archaeal sensory rhodopsin II from Natronomonas pharaonis, NpSRII, in complex with its transducer, NpHtrII. The NpSRII/NpHtrII complexes retained their structural integrity within SMA lipid particles as revealed by diverse biophysical techniques. Pulse electron paramagnetic resonance data showed the SMA-encased NpSRII/NpHtrII complexes as native-like 2 : 2 dimers with a distance between NpSRII sites 159 according to the “V”-shaped conformation found in a crystal structure.

Graphical abstract: Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles

Article information

Article type
Paper
Submitted
28 Sep 2017
Accepted
26 Oct 2017
First published
03 Nov 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 51324-51334

Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles

N. Voskoboynikova, W. Mosslehy, A. Colbasevici, T. T. Ismagulova, D. V. Bagrov, A. A. Akovantseva, P. S. Timashev, A. Y. Mulkidjanian, V. N. Bagratashvili, K. V. Shaitan, M. P. Kirpichnikov and H.-J. Steinhoff, RSC Adv., 2017, 7, 51324 DOI: 10.1039/C7RA10756K

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