Issue 6, 2017

Enrichment and fluorogenic labelling of 5-formyluracil in DNA

Abstract

Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated o-phenylenediamine directly tethered to naphthalimide can switch on 5-formyluracil, under physiological conditions, which can then be used in cell imaging after exposure to γ-irradiation. In addition, its labelling property caused the polymerase extension to stop in the 5-formyluracil site, which gave us more information than the fluorescence did itself. The idea of detecting 5-formyluracil might be used in the synthesis of other modified diaminofluoresceins.

Graphical abstract: Enrichment and fluorogenic labelling of 5-formyluracil in DNA

Supplementary files

Article information

Article type
Edge Article
Submitted
10 Feb 2017
Accepted
02 Apr 2017
First published
05 Apr 2017
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2017,8, 4505-4510

Enrichment and fluorogenic labelling of 5-formyluracil in DNA

C. Liu, Y. Wang, X. Zhang, F. Wu, W. Yang, G. Zou, Q. Yao, J. Wang, Y. Chen, S. Wang and X. Zhou, Chem. Sci., 2017, 8, 4505 DOI: 10.1039/C7SC00637C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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