A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells

Naseer Ullah Khan; Hua He; Xiaojuan Wang; Baosheng Ge; Qian Wang; Xu Liu; Jun Lao; Yanan Wang; Jiqiang Li; Zhencai Wang; Shitan Zhou; Fang Huang

doi:10.1039/C7CC08167G

A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells†

Naseer Ullah Khan,^a Hua He,

*^a Xiaojuan Wang,^a Baosheng Ge,^a Qian Wang,^a Xu Liu,^a Jun Lao,^a Yanan Wang,^a Jiqiang Li,^a Zhencai Wang,^a Shitan Zhou^a and Fang Huang

*^a

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* Corresponding authors

^a State Key Laboratory of Heavy Oil Processing and College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China
E-mail: huahe@upc.edu.cn, fhuang@upc.edu.cn

Abstract

An ultra-high fluorescence enhancement for two dyes on photonic crystal films was achieved to construct a two-color immuno-dot blot assay. This assay was demonstrated to simultaneously detect chemokine receptor co-expressed in cancer cells and reveal their co-operative and subtle changes after binding with respective ligands and drugs.

Chemical Communications

A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells†

Abstract

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A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells

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