Issue 11, 2018

‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes

Abstract

β(1,4)-Galactosyltransferase (β4Gal-T1) and T. cruzi trans-sialidase (TcTS) have been used in a ‘one-pot’ cascade to provide vesicles (liposomes) with a trisaccharide coating. These soluble enzymes catalysed the transfer of galactose then sialic acid onto a synthetic N-acetylglucolipid embedded in the bilayers. Clustering of this substrate into microdomains increased the rate of sialylated lipid production, showing that an increase in β4Gal-T1 activity is carried through the enzymatic cascade. These coatings modulated cell recognition. Hepatocellular carcinoma cells took up vesicles modified by β4Gal-T1 alone more extensively than sialylated vesicles produced by ‘one-pot’ sequential enzymatic modification.

Graphical abstract: ‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes

Supplementary files

Article information

Article type
Communication
Submitted
28 Nov 2017
Accepted
04 Jan 2018
First published
04 Jan 2018

Chem. Commun., 2018,54, 1347-1350

‘One-pot’ sequential enzymatic modification of synthetic glycolipids in vesicle membranes

F. L. Craven, J. Silva, M. D. Segarra-Maset, K. Huang, P. Both, J. E. Gough, S. L. Flitsch and S. J. Webb, Chem. Commun., 2018, 54, 1347 DOI: 10.1039/C7CC09148F

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