Issue 14, 2018
From the journal:

Chemical Communications

An allylated firefly luciferin analogue with luciferase specific response in living cells†

Yuma Ikeda,a   Tsuyoshi Saitoh,b   Kazuki Niwa,c   Takahiro Nakajima, ORCID logo d   Nobuo Kitada,e   Shojiro A. Maki, ORCID logo e   Moritoshi Sato,d   Daniel Citterio, ORCID logo *a   Shigeru Nishiyamaf  and  Koji Suzuki*a  

* Corresponding authors

a Department of Applied Chemistry Faculty of Science and Technology, Keio University, Hiyoshi 3-14-1, Kohoku-ku, Yokohama, Kanagawa, Japan
E-mail: citterio@applc.keio.ac.jp, suzuki@applc.keio.ac.jp

b International Institute for Integrative Sleep Medicine, University of Tsukuba, Tennodai 1-1-1, Tsukuba-shi, Ibaraki, Japan

c National Institute of Advanced Industrial Science and Technology, Tsukuba-shi, Ibaraki, Japan

d Graduate School of Arts and Science, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan

e Department of Engineering Science, Graduate School of Informatics and Engineering, University of Electro-Communications, Chofu, Tokyo, Japan

f Department of Chemistry, Faculty of Science and Technology, Keio University, Hiyoshi 3-14-1, Kohoku-ku, Yokohama, Kanagawa, Japan

Abstract

An allylated firefly luciferin was successfully synthesized and its bioluminescence properties were evaluated. When applied to cellular imaging in combination with Eluc, which is one of the commercially available luciferases, this analogue displayed a luciferase-specific bioluminescence signal with prolonged emission (>100 min).

Graphical abstract: An allylated firefly luciferin analogue with luciferase specific response in living cells

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Article information

DOI
https://doi.org/10.1039/C7CC09720D
Article type
Communication
Submitted
20 Dec 2017
Accepted
19 Jan 2018
First published
24 Jan 2018

Chem. Commun., 2018,54, 1774-1777

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An allylated firefly luciferin analogue with luciferase specific response in living cells

Y. Ikeda, T. Saitoh, K. Niwa, T. Nakajima, N. Kitada, S. A. Maki, M. Sato, D. Citterio, S. Nishiyama and K. Suzuki, Chem. Commun., 2018, 54, 1774 DOI: 10.1039/C7CC09720D

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