A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells

Radhika Mehta; Munaum H. Qureshi; Meredith K. Purchal; Sylvester M. Greer; Shanzhong Gong; Chinh Ngo; Emily L. Que

doi:10.1039/C8CC02034E

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A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells†

Radhika Mehta,

^a Munaum H. Qureshi,^a Meredith K. Purchal,^a Sylvester M. Greer,^a Shanzhong Gong,^b Chinh Ngo^a and Emily L. Que

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Texas at Austin, 105 E 24th St Stop A5300, Austin, TX 78712, USA
E-mail: emilyque@cm.utexas.edu

^b Department of Molecular Biosciences, University of Texas at Austin, 2506 Speedway, Austin, TX 78712, USA

Abstract

We report the synthesis and application of a small molecule probe for carbonic anhydrase (CA) to track holo-CA in cell lysates and live-cell models of zinc dyshomeostasis. The probe displays a 12-fold increase in fluorescence upon binding to bovine CA and also responds to human CA isoforms.

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Article information

DOI: https://doi.org/10.1039/C8CC02034E
Article type: Communication
Submitted: 13 Mar 2018
Accepted: 01 May 2018
First published: 01 May 2018

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Chem. Commun., 2018,54, 5442-5445

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A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells

R. Mehta, M. H. Qureshi, M. K. Purchal, S. M. Greer, S. Gong, C. Ngo and E. L. Que, Chem. Commun., 2018, 54, 5442 DOI: 10.1039/C8CC02034E

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Chemical Communications

A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells†

Abstract

Supplementary files

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A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells

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