Issue 43, 2018
From the journal:

Chemical Communications

Enzymatic biofuel cell-based self-powered biosensing of protein kinase activity and inhibition via thiophosphorylation-mediated interface engineering†

Chengcheng Gu,‡a   Panpan Gai,‡a   Lei Han, ORCID logo a   Wen Yu,a   Qingyun Liub  and  Feng Li ORCID logo *a  

* Corresponding authors

a College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, P. R. China
E-mail: lifeng@qust.edu.cn

b College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, P. R. China

Abstract

We developed a facile and ultrasensitive enzymatic biofuel cell (EBFC)-based self-powered biosensor of protein kinase A (PKA) activity and inhibition via thiophosphorylation-mediated interface engineering. The detection limit was down to 0.00022 U mL−1 (S/N = 3). In addition, the PKA activities from MCF-7 and A549 cell lysates were analyzed and achieved reliable results.

Graphical abstract: Enzymatic biofuel cell-based self-powered biosensing of protein kinase activity and inhibition via thiophosphorylation-mediated interface engineering

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Article information

DOI
https://doi.org/10.1039/C8CC02328J
Article type
Communication
Submitted
23 Mar 2018
Accepted
01 May 2018
First published
01 May 2018

Chem. Commun., 2018,54, 5438-5441

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Enzymatic biofuel cell-based self-powered biosensing of protein kinase activity and inhibition via thiophosphorylation-mediated interface engineering

C. Gu, P. Gai, L. Han, W. Yu, Q. Liu and F. Li, Chem. Commun., 2018, 54, 5438 DOI: 10.1039/C8CC02328J

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