Issue 57, 2018
From the journal:

Chemical Communications

Energy landscape of polymorphic amyloid generation of β2-microglobulin revealed by calorimetry†

Misaki Kinoshita,‡a   Yuxi Lin,‡b   Itoh Dai,a   Masaki Okumura,c   Natalia Markova,d   John E. Ladbury,e   Fabio Sterponef  and  Young-Ho Lee ORCID logo *a  

* Corresponding authors

a Institute for Protein research, Osaka University, Yamadaoka 3-2, Suita, Osaka 565-0871, Japan
E-mail: mr0505@protein.osaka-u.ac.jp

b Department of Chemistry, Sookmyung Women's University, Cheongpa-ro 47-gil 100, Yongsan-gu, Seoul, Korea

c Frontier Research Institute for Interdisciplinary Sciences (FRIS), Tohoku University, 6-3 Aramaki-Aza-Aoba, Aoba-ku, Sendai 980-8578, Japan

d Malvern Panalytical, 22 Industrial Drive East, Northampton, MA, USA

e School of Molecular and Cellular Biology, University of Leeds, Leeds, UK

f CNRS Laboratoire de Biochimie Théorique, Institut de Biologie Physico-Chimique, Université Paris Denis Diderot, Sorbonne Paris Cité, PSL Research University, 13 rue Pierre et Marie Curie, Paris, France

Abstract

Understanding of amyloid aggregation in terms of thermodynamics and kinetics is still limited. We herein examined the mechanism of β2-microglobulin amyloidogenesis using our unique method of isothermal titration calorimetry-based thermodynamic/kinetic measurements, and revealed the energy landscape of polymorphic amyloidogenesis under biological environment-mimicking conditions including shear forces and crowding effects.

Graphical abstract: Energy landscape of polymorphic amyloid generation of β2-microglobulin revealed by calorimetry

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Article information

DOI
https://doi.org/10.1039/C8CC02718H
Article type
Communication
Submitted
04 Apr 2018
Accepted
26 Jun 2018
First published
27 Jun 2018

Chem. Commun., 2018,54, 7995-7998

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Energy landscape of polymorphic amyloid generation of β2-microglobulin revealed by calorimetry

M. Kinoshita, Y. Lin, I. Dai, M. Okumura, N. Markova, J. E. Ladbury, F. Sterpone and Y. Lee, Chem. Commun., 2018, 54, 7995 DOI: 10.1039/C8CC02718H

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