Issue 56, 2018

Charge designable and tunable GFP as a target pH-responsive carrier for intracellular functional protein delivery and tracing

Abstract

A charge designable and tunable green fluorescent protein (GFP)-based protein delivery strategy was proposed. The acquired His29GFP selectively permeates the cell membrane at a target pH of 6.5 and escapes from the endosome efficiently. The delivered RNase A caused substantial mRNA degradation in HeLa cells, and proliferation inhibition in different cell lines and a 3D tumor model at pH 6.5.

Graphical abstract: Charge designable and tunable GFP as a target pH-responsive carrier for intracellular functional protein delivery and tracing

Supplementary files

Article information

Article type
Communication
Submitted
23 Apr 2018
Accepted
11 Jun 2018
First published
12 Jun 2018

Chem. Commun., 2018,54, 7806-7809

Charge designable and tunable GFP as a target pH-responsive carrier for intracellular functional protein delivery and tracing

S. Hu, X. Chen, C. Lei, R. Tang, W. Kang, H. Deng, Y. Huang, Z. Nie and S. Yao, Chem. Commun., 2018, 54, 7806 DOI: 10.1039/C8CC03285H

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