Issue 73, 2018

Coupling oligonucleotides possessing a poly-cytosine tag with magnetic ionic liquids for sequence-specific DNA analysis

Abstract

Oligonucleotide probes were designed with a poly-cytosine region that facilitates stable anchoring to a magnetic ionic liquid support. By tethering a recognition sequence to the poly-C tag, the resulting diblock oligonucleotides distinguished single-nucleotide variants and captured DNA targets from interfering genomic DNA and cell lysate for qPCR amplification.

Graphical abstract: Coupling oligonucleotides possessing a poly-cytosine tag with magnetic ionic liquids for sequence-specific DNA analysis

Supplementary files

Article information

Article type
Communication
Submitted
23 Jul 2018
Accepted
13 Aug 2018
First published
13 Aug 2018

Chem. Commun., 2018,54, 10284-10287

Author version available

Coupling oligonucleotides possessing a poly-cytosine tag with magnetic ionic liquids for sequence-specific DNA analysis

X. Peng, K. D. Clark, X. Ding, C. Zhu, M. Varona, M. N. Emaus, J. An and J. L. Anderson, Chem. Commun., 2018, 54, 10284 DOI: 10.1039/C8CC05954C

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