Tracking mitochondrial 1O2-ROS production through a differential mitochondria-nucleoli fluorescent probe

Abstract

The dual-emissive fluorescent probe described here enabled dynamic tracking of singlet oxygen (¹O₂)-ROS species production and localization dynamics between the mitochondria and nucleoli in the presence of agents perturbing the mitochondrial membrane potential and under a photodynamic (PDT) system. Local structural information during the probe-¹O₂ interaction was followed by spectrally resolved confocal microscopy.